The ultimate aim of biocatalysis research is to replace the petrochemical industry with clean and green microbial reactions using renewable and non-toxic feedstocks. But how exactly do we go about replacing industrial chemical reactions with biochemical reactions? How can we use microbes and enzymes to make the useful chemicals that we need in our daily lives?
We are especially interested in the reactions of monooxygenases - these enzymes add oxygen to specific locations in organic modules, yielding alcohols and epoxides. Some monooxygenases act in a highly selective fashion, e.g. making nearly pure single enantiomers of epoxides, which are valuable for pharmaceutical synthesis. Our favourite enzyme is the alkene monooxygenase of Mycobacterium spp. This fascinating enzyme can do tricks that organic chemists can only dream of, but annoyingly, it is found in a bug that is very hard to work with. Genetic engineering to the rescue! This project will aim to express the ethene monooxygenase and related interesting enzymes in more industrially-useful hosts (e.g. E.coli). We will investigate issues like codon usage, accessory proteins, growth conditions, different cloning vectors and cloning hosts in an attempt to come up with a high-activity and easy-to-use recombinant system for converting alkenes to epoxides
The project requires skills and knowledge in microbiology, molecular biology, chemistry and biochemistry. However if you can meet some of these requirements we can teach you others as part of your PhD training.
HDR Inherent Requirements
In addition to the academic requirements set out in the Science Postgraduate Handbook, you may be required to satisfy a number of inherent requirements to complete this degree. Example of inherent requirement may include:
The opportunity ID for this research opportunity is 2834