The new discipline of synthetic biology (SynBio) promises to transform every aspect of our lives, from medicines to agriculture to industry. The vision of SynBio is that organisms can be created in a predictable way from genetic building blocks, just like Lego. The reality does not always live up to this vision, due to the complexities of living systems. How can we better employ these powerful tools to provide the maximum benefits to society?
With the constantly decreasing costs of de novo DNA synthesis, new possibilities are constantly opening up for synthetic biology. It is becoming easier and easier to design genes, gene clusters, and even whole organisms from scratch. To accelerate this research, we see a need for new plasmid-based cloning tools that are compatible with modern methods (e.g. Golden Gate assembly) and take advantage of new discoveries in promoter systems, marker genes etc. We aim to develop versatile cloning plasmids which will enable gene expression and knockouts to be made in several key bacterial genera which are important as 'workhorse' organisms, and/or relevant to agriculture, industry or medicine, such as E.coli, Pseudomonas and Mycobacterium spp.
This project will require a background in microbiology and molecular biology.
HDR Inherent Requirements
In addition to the academic requirements set out in the Science Postgraduate Handbook, you may be required to satisfy a number of inherent requirements to complete this degree. Example of inherent requirement may include:
The opportunity ID for this research opportunity is 2836