Whole genome CRISPR screening to define drug mechanism of action

Summary

This project will involve new whole genome CRISPR editing strategies, coupled with bioinformatics analysis, to identify the complete molecular mechanism of action for relevant human cancer drugs or toxins. 

Supervisor(s)

Professor Greg Neely

Research Location

Charles Perkins Centre - Greg Neely Lab

Program Type

PHD

Synopsis

Recently CRISPR-based approaches have revolutionized functional genomics techniques. We have set up new whole genome CRISPR screening, and using this technique we have defined the molecular mechanism of action for over 20 relevant human cancer drugs or venoms from deadly Australian creatures. This project involves working with our functional genomics team to continue these efforts. The successful candidate will learn state of the art whole genome CRISPR screening, as well as advanced molecular biology and potentially also basic bioinformatics analysis. This project involves testing human genomics data on longevity to find new genes that can extend lifespan in the fruit fly. We focus on genes that when targeted specifically in the brain, can extend lifespan while also preserve cognitive function with age. We have identified 30 new targets that can extend lifespan, and the project will involve working on some of these new lifespan extending genes. Project will involve lifespan assays, behavioral genetics, molecular biology, and electrophysiology, and the successful candidate will be trained in all of these techniques.

 Exceptional applicants may qualify for an additional elite top-up scholarship worth up to $10000 per year.

Additional Information

HDR Inherent Requirements

In addition to the academic requirements set out in the Science Postgraduate Handbook, you may be required to satisfy a number of inherent requirements to complete this degree. Example of inherent requirement may include:

- Confidential disclosure and registration of a disability that may hinder your performance in your degree;
- Confidential disclosure of a pre-existing or current medical condition that may hinder your performance in your degree (e.g. heart disease, pace-maker, significant immune suppression, diabetes, vertigo, etc.);
- Ability to perform independently and/or with minimal supervision;
- Ability to undertake certain physical tasks (e.g. heavy lifting);
- Ability to undertake observatory, sensory and communication tasks;
- Ability to spend time at remote sites (e.g. One Tree Island, Narrabri and Camden);
- Ability to work in confined spaces or at heights;
- Ability to operate heavy machinery (e.g. farming equipment);
- Hold or acquire an Australian driver’s licence;
- Hold a current scuba diving license;
- Hold a current Working with Children Check;
- Meet initial and ongoing immunisation requirements (e.g. Q-Fever, Vaccinia virus, Hepatitis, etc.)

You must consult with your nominated supervisor regarding any identified inherent requirements before completing your application.

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Keywords

whole Genome CRISPR, Deep sequencing, Cancer, Emergency medicine, Bioinformatics

Opportunity ID

The opportunity ID for this research opportunity is: 2038

Other opportunities with Professor Greg Neely