Develop single-molecule and super-resolution microscopy imaging technologies in the cardiovascular disease

Summary

This project will focus on development, improvement, and application of single-molecule tracking and super-resolution imaging, such as TIRF, HiLo, PALM, STORM, Lattice Light-Sheet Microscopy and other technologies, for the analysis of key proteins in the process of thrombosis and platelet activation. We are collaborating with Nobel Laureate Eric Betzig’s team members at UC Berkeley

Supervisor(s)

Dr Lining (Arnold) Ju

Research Location

Biomedical Engineering - BMIT, School of Physics, Brain and Mind Centre

Program Type

Masters

Synopsis

Dynamic processes such as molecular conformational changes (i.e. integrin receptors, cytoskeletons, and mechano-sensitive ion channels), protein assembly and relocation, and protein-protein interactions. We aim to make technological breakthroughs in time, space and multiple dimensions, and draw the platelet "molecular interactome" of healthy people and cardiovascular patients with diabetes, obesity and metabolic syndromes. Finally, the high-volume 2D-3D image data analyses are combined for anti-platelet drug screenings.

Additional Information

• Current PHD and/or Masters topics 1) Nature Materials (2019) –Chen Y#, Ju LA#, Zhou F, Liao J, Xue L, Yuan Y, Su QP, Jin D, Lu H, Jackson SP, and Zhu C (2019). An integrin αIIbβ3 intermediate affinity state mediates biomechanical platelet aggregation. Nature Mat 18(7): 760-769 doi: 10.1038/s41563-019-0323-6 Commented by Nature Materials (18(7):661–662) in the same issue. [IF 39.74] https://sydney.edu.au/news-opinion/news/2019/04/02/research-unlocks-biomechanic-mystery-behind-deadly-blood-clots.html  2) Nature Communications (2018) – Ju L, McFadyen JD, Al-Daher S, et al. (2018) Compression force sensing regulates integrin αIIbβ3 adhesive function on diabetic platelets. Nature Commun 9(1): 1084. doi: 10.1038/s41467-018-03430-6 [IF 12.53] https://www.hri.org.au/news/blood-clot-breakthrough-a-saviour-for-diabetics 
Eligibility criteria / candidate profile You will have: 1) Skilled in using at least one optical design software such as ZEMAX, Lighttools, Codev, TRACEPRO;  2) Master basic optical theory, diffractive optics, Fourier optics, photoelectric information processing and other basic theories, and understand the design principles of microscope imaging systems;  3) Familiar with the design of optoelectronic system architecture. Experience in microscope and imaging optical system design and production is preferred.  4) Responsible for optical system design and component selection, assembly and debugging of optical systems;  5) Solid basic knowledge of biology and rich experience in the PC2 biological laboratory, applicants with related scientific backgrounds such as pathological imaging diagnosis, intracellular organ imaging mechanism analysis, bioprobe labeling, targeted therapy, etc.
Preferred experience include: 1) Optical microscopy imaging, optical super-resolution imaging, adaptive optics, the principles and characterization of fluorescent materials, and the principle of photon matter interaction;  2) Using spatial light modulator, deformable mirror device, and acoustic optical deflector.
 • Scholarship(s) / funding available 1) ARC Discovery Project DP200101970 (CI-A) “Integrin Activation by Fluid Flow Disturbance: Mechanobiology Approaches”  2) ARC Linkage Infrastructure, Equipment and Facilities (co-CI) LE190100130 “Volumetric Imaging Facility: Observing the Cell in its Native Environment”.  3) Sydney Local Health District - Annual Health Research Infrastructure Award (AHRIA). Conferred by NSW Minister for Health (https://www.slhd.nsw.gov.au/sydneyresearch/pdf/news42.pdf)

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Keywords

super-resolution microscopy, STORM, TIRF, Single molecule, Biophysics, ion channel, membrane, receptors, mechanobiology, microfluidics, cardiovascular, Stroke, Platelet, Thrombosis, Piezo, Red blood cell, diabetes

Opportunity ID

The opportunity ID for this research opportunity is: 2786

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