About Dr Jingjing You

My research interests lie on developing novel corneal disease treatments which include the following three main areas: 1. corneal bioengineering which combines generation of novel biomaterial and 3D cell printing to facilitate wound healing, 2. gene editing on cells to treat genetic affected corneal diseases, and 3. Development of electromechanical treatment for corneal diseases.

Cell biology, 3D cell printing, molecular biology, next generation gene sequencing, human cornea diseases, in vitro and in vivo model studies

After working on corneal research for just over 5 years, I have published more than 20 papers with 124 citations; attracted over $1M in competitive fundings, and established collaborations with major universities including UNSW, UniMelb and QUT. I am visiting scholar at UNSW, a committee member of Human Eye Cell Atlas Consortium, and an organiser for annual Australia corneal bioengineering working group. I have also been invited as keynote speaker in multiple conferences, an external reviewer for NHMRC grant, a reviewer for multiple international peer-review journals, and as a member of judge panels for HDR student presentations in multiple occations.Key developments generated from my corneal research include: 
1. Initiated the RNA sequencing research on myopic and keratoconus corneal epithelial cells, and the results led to a publication and the first public available genetic database for corneal epithelium on https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE112155.

2. As one of the pioneering groups analysing the human tear proteome. I firstly investigated the phosphor and glycol-protein profiles in human tears in 2010 and demonstrated the use of multiple reaction monitor (MRM) techniques to validate and quantify proteins. The results have generated multiple publications with a combined of over 50 citations.

3. Development of novel biomaterials for corneal regeneration. With the understanding of cornea structure and cell biology, I lead research projects on the development of novel biomaterials for corneal healing. Through collaboration with Intelligent Polymer Research Institute, University of Wollongong, a novel biomaterial was developed, which lead to a provisional patent.

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Selected publications

1. You J, Corley SM, Wen L, Hodge C, Hollhumer R, Madigan MC, et al. RNA-Seq analysis and comparison of corneal epithelium in keratoconus and myopia patients. Scientific reports. 2018;8(1):389.
2. You J, Munoz-Erazo L, Wen L, Hodge C, Madigan MC, Sutton G. In-Vitro Effects of Secreted Frizzled-Related Protein 1 (SFRP1) On Human Corneal Epithelial Cells. Current eye research. 2018; 43(4):455-9.
3. Chen Z, You J, Liu X, Cooper S, Hodge C, Sutton G, et al. Biomaterials for corneal bioengineering. Biomedical materials. 2018;13(3):032002.
4. Pinheiro L, O`Brien H, Druce J, Do H, Kay P, Daniels M, You J, Burke D, Griffiths K, Emslie K. (2017) Interlaboratory Reproducibility of Droplet Digital Polymerase Chain Reaction Using a New DNA Reference Material Format. Analytical Chemistry, 89:11243-11251.
5. Markoulli M, You J, Kim J, Duong CL, Tolentino J, Karras J, Lum E. (2017) Corneal nerve morphology and tear film substance P in diabetes. Optometry and Vision Science (Accepted).
6. Markoulli M, Gokhale M, You J. (2017) Substance P in Flush Tears and Schirmer Strips of Healthy Participants. Optometry and Vision Science; 94:527-533.
7. Golebiowski B, Badarudin N, Eden J, Liu J, Gerrand L, Robinson J, Hampel U, You J, Stapleton F. (2016) The effects of transdermal testosterone and oestrogen therapy on dry eye in post-menopausal women: a randomised, placebo-controlled, pilot study. British Journal of Ophthalmology (Epub ahead). doi: 10.1136/bjophthalmol-2016-309498.
8. You J, Willcox M, Fitzgerald A, Schiller B, Cozzi PJ, Russell PJ, Walsh BJ, Wasinger VC, Graham PH, Li Y. (2016) Absolute quantification of human tear lactoferrin using multiple reaction monitoring technique with stable-isotopic labeling. Analytical biochemistry; 496:30-4.
9. Golebiowski B, Badarudin N, Eden J, You J, Hampel U, Stapleton F. Does endogenous serum oestrogen play a role in meibomian gland dysfunction in postmenopausal women with dry eye? (2016) British Journal of Ophthalmology. Doi: 10.1136/bjophthalmol-2016-308473
10. You J, Wen L, Roufas A, Hodge C, Sutton G, Madigan MC. (2015) Expression of HGF and c-Met Proteins in Human Keratoconus Corneas. Journal of ophthalmology. 2015:852986.