The Spatial Pan-Omics Initiative leverages the capabilities and expertise of our Sydney Mass Spectrometry and Sydney Cytometry facilities to deliver one of the most extensive and integrated spatial multi‑omics platform collections in Australia.
Integrating cutting-edge mass spectrometry and spatial transcriptomics platforms, we enable unprecedented insight into the molecular and cellular architecture of biological systems.
Situated within the Charles Perkins Centre, we provide exceptional technical support and data analysis capabilities to enable researchers and industry to address complex questions in health, disease, and environmental science.
Our experts help you get the most from our state–of-the-art tools and techniques.
Dr Thomas Ashhurst, Senior Cytometry Scientist
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Thomas Ashhurst PhD is a Senior Cytometry Scientist with the Sydney Cytometry Core Research Facility. In this role he specialises in the use of cutting edge high-dimensional cytometry, single-cell, and spatial multiomics technologies for interrogating cellular systems. His work involves the development of new computational and experimental methods to better understand immune development and pathology across the human lifespan. He holds a long-standing interest in the pathogenesis of severe respiratory and neuroinvasive infectious diseases.
Jason Madore, Senior Cytometry Specialist (Spatial Biology)
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Jason Madore is a Spatial Biology Specialist at the Sydney Cytometry Core Research Facility, where he works on spatial and multiplexed imaging workflows for high-plex immunofluorescence and image-based spatialomics. He provides analytical support to researchers through the planning and implementation of spatial biology experiments and the computational analysis pipelines used to analyse complex spatial datasets.
Jason holds an MSc from the Université de Montréal and has a background in translational/pathobiology cancer research. He specialises in computational spatial analysis, including spatial image processing, neighbourhood and proximity modelling, cell–cell interaction analysis, and quantitative spatial statistics. He develops and applies custom Python and R pipelines for spatial proteomic and transcriptomics data, supporting analysis of tissue architecture, cellular organisation, and tumour microenvironment characterisation.
Dr Alex Rookyard, Senior Technical Officer
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Alex has over eight years' experience in proteomic, metabolomic, and lipidomic analysis. He started his journey with mass spectrometry at the University of Sydney where during his PhD he developed methods to quantify irreversible post-translational modification to cysteine residues by targeted mass spectrometry. After this Alex spent two years furthering his proteomic capabilities at Columbia University, New York, where he conducted large-scale analysis of cerebral spinal fluid in the context of Alzheimer’s disease. He is now the leading expert on the only Illumina Protein Prep Platform in the Southern hemisphere.
Dr Jessica Pandohee, Senior Technical Officer
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Jess supports our laser-capture microdissection workflows and holds a Bachelor of Forensic Science (Deakin University, 2012) and a PhD in Applied Chemistry (RMIT University, 2017). Her PhD research involved the development of multidimensional chromatographic techniques for targeted and untargeted study of metabolites and its application in the field of food, agriculture and forensic science. The work for which she received the RMIT Vice Chancellor Prize for Research Excellence, The Agilent Technologies Award for Separation Science and an Honourable Mention for the IUPAC-Solvay award in 2018. Prior to joining the Sydney Mass Spectrometry, Jess completed a post-doctoral fellowship at the Centre for Integrative Metabolomics and Computational Biology (Edith Cowan University) and the Centre for Crop and Disease Management (Curtin University). Jess’ interests in improving human health and tackling global challenges arising from a growing food, environmental and water insecurity have led her in collaborative projects across Australia and internationally.
Dr Olivia Lee, Senior Technical Officer
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Olivia completed her PhD at the University of South Australia, where she investigated tissue-specific glycomic changes using mass spectrometry imaging. In 2023, she joined Uppsala University, Sweden, as a postdoctoral researcher, where she expanded her expertise in mass spectrometry imaging and LC-MS/MS-based proteomics through involvement in a wide range of core-facility projects. With over 10 years of research experience, Olivia specialises in LC-MS-based proteomics and MALDI mass spectrometry imaging for lipids, glycans and peptides, with a focus on untargeted biomarker discovery. She is skilled in tissue processing, histological staining, data analysis and method development for multi-omics integration. Her research interests include sample optimisation for mass spectrometry imaging and discovery-driven proteomics.
Dr Samiuela Lee, Senior Technical Officer
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With over 15 years of experience in mass spectrometry, Sam graduated with first-class honours in medical science and earned a PhD in analytical chemistry with a focus on LC-PDA-MS/MS method validation for complex herbal formulations from Wester Sydney University (WSU) in 2010. Post-PhD his career has encompassed roles such as a Laboratory Manager at a TGA certified laboratory at the herbal analysis lab at the National Institute for Complementary Medicine (NICM), WSU, Research Scientist for the Kids Cancer Alliance (KCA) within Westmead Children's Hospital, Project Scientist at the National Measurement Institute (NMI), and Research Associate at the School of Chemical and Biomolecular Engineering before joining Sydney Mass Spectrometry.
Sam's core expertise revolves around method development and validation of targeted metabolomic LC-MS/MS methods in diverse fields including phytochemical, pharmaceutical, pesticide, environmental, and clinical research. He has a proven track record in supporting users in discovery workflows utilising orbitrap and TOF MS instruments.
Integrating spatial transcriptomics, proteomics, metabolomics and high‑parameter imaging, reveals how cells, molecules and pathways are organised and function within intact tissues. Access to these capabilities is transforming research by revealing how cells, molecules, and biological processes function within their native environments, and enabling deeper insights that guide discovery and therapeutic innovation. See our services below.
Use the CosMx or Xenium systems for targeted spatial transcriptomics or switch to the Visium for spatially resolved whole transcriptome gene expression profiling combined with histology.
The PhenoImager HT and Lunaphore COMET can be used to do high-plexed spatial Proteomics analysis imaging up to 40 proteins at time.
Use the Orbitrap Astral Zoom Mass Spectrometer to dive into the proteome of individual cells. Through a partnership with the ACRF single cell proteomics laboratory we have a comprehensive facility that can cut individual cells from tissue sections or array cell suspension into 384 well plates and then process ready for analyse by mass spectrometry. It is possible to get 5000 proteins per cell at a rate of over 100 cells per day.
The aptamer (aka SOMAmers) technology in the Illumina Protein Prep (IPP) can profile over 9500 proteins in human plasma or serum. Using precision robotics, two rounds of purification are done before the aptamers are barcode with short oligonucleotides and quantified using next-generation sequencing. The assay is designed around a 96 well plate format with 85 samples and 11 controls that allow for cross plate, run and facility comparisons, for plasma samples the median intra-run CV is on 4.7%, and inter-run CVs are less 5.8%.
Mass spectrometry imaging (MSI) enables the detection and high-resolution visualisation of biomolecules, such as lipids, drugs, glycans or proteins, in tissue sections of your sample of interest. The molecules can be ionised either by Desorption Electro Spray Ionisation (DESI) or by Matrix Assisted Laser Desorption/Ionisation MALDI before being analysed by mass spectrometry.
Location
Spatial Pan-Omics Initiative is located in the Charles Perkins Centre.
Delivery address
Sydney Mass Spectrometry
via The Hub Loading Dock
Charles Perkins Centre (D17)
Orphans School Creek Lane
Camperdown
University of Sydney, NSW, 2006
Dock receiving time: 08:00 – 15:00, Mon - Fri.