The multi-user advanced microscopy facility at the Bosch Institute provides access to, and expert training on basic transmitted light and fluorescence microscopes, advanced confocal microscopes and the PALM Combi System that combines laser microdissection pressure catapulting and optical tweezers in one instrument. Registered users also have access to image analysis and processing software such as MetaMorph and Bitplane Imaris Suite.
Book facility: registration form
Location: Room S443, Anderson Stuart Building
Sadaf Kalam, Facility Officer
Ph: 9351 2520
Mohammad Nasir Uddin, Microscopy Officer
Ph: 9351 2520
Chad Moore, Microscopy Officer
Ph: 9351 2520
Nuance Multi-Spectral Imaging System (Room N421)
This Nuance camera allows multi-spectral data acquisition using a liquid crystal tunable filter that provides fast, accurate spectral imaging from 420 to 720 nm (VIS) and 500 to 950 nm (NIR), covering a range of available dyes and fluorophores.
Olympus MBF/Stereo Investigator Transmitted Light Microscope (Room N421)
The Virtual Slice module can automatically create a montage by collecting a series of contiguous images from a tissue specimen. The software aligns, stitches and blends images into a seamless montage. This microscope is set up for bright-field imaging and enables fast navigation to any region of the slide.
Zeiss "Deconvolution" Microscope (Room S443)
This microscope has a motorized transmitted-light beam path and focus drive and can be operated via a touch-sensitive TFT display, by pressing buttons directly on the microscope or via the ZEN (ZEISS Efficient Navigation) software. The system is ideal for imaging specimens using different transmitted-light contrasting and fluorescence techniques with reproducible settings.
ZEISS LSM 800 Confocal Microscope plus Airyscan (Room S448)
This confocal microscope is equipped with 405, 488, 561, 640 nm laser lines, the Airyscan super-resolution module plus two individual GaAsP PMT detectors, a transmitted light detector and an AxioCam 506 digital camera.
Zeiss LSM 510 Meta Spectral Confocal Microscope (Room S448)
This spectral confocal microscope is based on an inverted Zeiss AxioObserver microscope. It has a number of lasers for excitation of fluorescent dyes and fluorescent proteins.
Leica SPE-II Confocal Microscope (Room S443)
This high resolution spectral confocal microscope is compact and easy-to-use. It consists of four solid-state lasers (405 nm, 488 nm, 532 nm and 635 nm) that allows a broad spectrum of dyes to be imaged.
PALM DuoFlex Combi System (Room N135)
The PALM DuoFlex Combi System is an integrated laser microdissection, optical tweezers and microscope system for live cells and fixed material.
The Bosch Institute’s Animal Behavioural Facility (ABF) is a centre for researchers working with rodents, with a focus on behavioural analyses. The facility offers services and access to sophisticated behavioural testing equipment and provides expertise for all aspects of experimental design, execution and analyses, as well as novel behavioural solutions and ongoing support.
Book equipment: registration form
Thomas Burton, Facility Research Officer
Ph: 9114 1431
The IntelliCage (NewBehavior, TSE Systems) is a platform for the automated testing of mouse activity and behaviour in the home cage and is ideal for phenotyping genetically modified mice, profiling animal models of diseases/disorders and screening effects caused by the administration of drugs, surgery or other interventions.
This top-view based behaviour recognition software (CleverSys Inc.) automatically analyses videos of behavioural tasks that are filmed from above (e.g. mazes, open field activity, novel object recognition, conditioned place preference etc.).
Bussey-Saksida Touchscreen Operant Chambers
This array of 4 operant chambers utilises touchscreen technology and allows laboratory mice to interact directly with a device that is very similar to our very own smartphone or tablet.
Gait Analysis System
This complete gait analysis package (Clever Sys Inc.), which includes a customised treadmill and GaitScan software, is a highly sensitive and detailed means of investigating motor coordination and function in animal models of a range of pathophysiological conditions.
Conditioned Place Preference (CPP) & Fear Conditioning
CPP (Clever Sys Inc.]]) is commonly used in preclinical stages to determine the rewarding and aversive effects of drugs. It may also be used to examine aspects of learning and memory, preference of other stimuli (e.g. food or copulation), reinforcement, Pavlovian conditioning etc.
The Rotarod (IITC Inc.) is a well-established means of assaying a number of motor functions in rodents such balance, coordination, motor learning and fatigue/endurance. Rotarod performance provides a sensitive index of motor dysfunction as a result of drug treatment, injury, surgical procedure, genetic manipulation etc.
Ultrasonic Vocalisation Analysis System
This equipment (Avisoft Bioacoustics) is high quality and automated means of analysing rodent Ultrasonic Vocalisations (USVs).
Our balance beam is a custom built apparatus for the study of balance and coordination in mice.
Grip Strength Meters
We have two digital forces gauges and accessories for testing muscle strength in rodent limbs. A trapeze-like horizontal bar (grasped by the forelimbs) or a grid (grasped by fore- and hind-limbs) attaches to a digital force gauge.
The multi-user Bosch Mass Spectometry Facility (BMSF) is a unique world-class facility for the detection, characterisation and quantification of biomarkers in cells, tissues and organs. The facility, which is PC2 certified, provides access to, and expert training in, equipment for state-of-the-art analytical mass analyses and mass mapping.
Location: G03A, Medical Foundation Building K25
Book equipment: booking form
Dr XiaoSuo Wang, Facility Officer
Ph: 9036 3460 (office); 9036 3060 (BMSF); 0423 335 188 (mobile)
Triple quadrupole mass spectrometer
Triple Quadrupole Mass Spectrometers are standard analytical tool for LC-MS/MS applications, in particular when accurate quantification is required. QQQ mass analysis is highly suitable for high throughput detection and accurate quantification of compounds, especially in complex biological matrices.
SHIMADZU LCMS-8050 (Triple Q)
The LCMS-8050 Triple Q mass spectrometer is featured for its UFsensitivity, UFswitching, UFscanning and UF-MRM.
Single quadrupole LC/MS
Agilent G6120B single quadrupole mass spectrometer was installed in 2012 and relocated at BMSF in Nov, 2013. Equipped with electrospray ionization (ESI) ion source, DAD and 1260 infinity LC series, the instrument is ideal to perform both qualitative and quatitative analysis on a range of samples including drugs, environmental contaminants, pharmaceuticals and peptides. The typical mass range is 1-3000 Da.
Other general equipment includes: a biological safetly cabinet; CO2 incubator; inverted microscope; centrifuges; concentrators; mini HPLC water system; shaking water bath; dry block heater and avaporator/ nitrogen blowing down system; tissue homogeniser; vacuum extraction manifold; balance; pH meter; freeze dryer; drying ovens; sonic water bath.
The Bosch Live Cell Analysis Facility (LCAF) is an open-access, multi-user facility that aims to provide all the necessary knowledge and instrumentation for its members to assess biological cell function. The facility provides expert training and access to live cell analysis and flow cytometry instruments, cryostats, equipment for protein and nucleic acid analysis, tissue culture facilities, centrifuges and general laboratory equipment. The LCAF also has a comprehensive Cell Bank that is available to registered users.
Book equipment: booking form
Ph: 9036 3223
Live cell analysis
Olympus IX83 Live Cell Imaging System
The EVOS FL AUTO System is a state-of-the-art live cell imaging system with its own incubator. The EVOS onstage incubator is an environmental chamber that enables precise control of temperature, humidity and three gases live cell imaging under both physiological and non-physiological conditions (such as hypoxia).
The Gallios Flow Cytometer is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection.
The BD FACSCalibur platform allows users to perform both cell analysis and cell sorting in a single bench-top system. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of lymphocyte subsets, stem cells, residual white blood cells, and reticulocytes.
Mac with FlowJo
An 27-inch iMac with FloJo is provided to analyse the data obtained with the FACSCalibur. The large screen size facilitates data analysis.
Nucelic acid analysis
Rotor Gene Q
The Rotor-Gene Q is a state-of-the-art innovative instrument that enables high-precision real-time PCR, end-point PCR, and high resolution melt (HRM) analysis. It is highly suited for use in gene expression analysis, genotyping, pathogen detection, and many other areas of research.
CAS-1200 automated PCR set up
The CAS-1200 is an easy to use robot developed specifically for the set up of real-time quantitative PCRs. The results achieved match or exceed the best hand pipetting.
Leica 1510S Cryostat
The cryostat is a rotary microtome contained in a mechanically refrigerated chamber that is used to cut sections of frozen tissues. The Leica CM1510 S is a powerful cryostat for routine as well as research applications in biology, medicine and industry. The instrument has been designed for rapid freezing and sectioning of tissue samples.
Olympus BX51 with DP70 camera
The OLYMPUS BX51 research microscope is a union of Olympus infinity corrected optical systems (UIS2 – universal infinity system) and advanced fluorescence or differential interference contrast DIC (Nomarski) technology.
The LCAF stores a wide variety of cell lines that are available to LCAF registered users. The LCAF also has all the necessary equipment for cell culture.
The Molecular Biology Facility (MBF) currently consists of six laboratories in the Anderson Stuart Building (F13) and Molecular Bioscience Building (G08). The Bosch Molecular Biology Facility in the Anderson Stuart Building was established in 2006. The Bosch Anderson Stuart PC2 Facility was officially open in September, 2011. The Bosch MBF PC2 facility in the Blackburn Building (D06) established in 2008 was relocated to the Molecular Bioscience Building (G08) in August, 2017.
Dr. Donna Lai, PhD Molecular Biology Officer
Ph: 9351 8787
Mob: 0403 151 568
Dr. Sheng Hua, PhD Molecular Biology Technical Officer
Ph: 9351 6026
Mob: 0405 382 568