Proteomics
Identifying and quantifying complex mixtures of proteins is a huge task. The best platform to use depends on the preferred method of quantitation. For example, Q Exactive HF-X and Exploris 480 platforms are popular for label free and SILAC analysis. While 6600 and 7600 TripleTOFs are preferred for SWATH and scanning SWATH analysis. The Tribrid mass spectrometers are recommended for optimal TMT labelled-based analysis.
Through the use of contemporary enrichment techniques, it is possible to identify and quantify tens of thousands of changes in the phosphoproteome. High throughput methods such as ‘EasyPhos’ are coupled with the latest Q Exactive platforms to create exceptional methods.
Glycomics is the characterisation of polysaccharides, typically derived from proteins. In glycopeptidomics, the aim is to identify both the sugar and the peptide while they are still linked together. This can be done the Fusion Lumos mass spectrometer which is equipped with Electron Transfer Dissociation and UVPD.
Top-down and intact protein analysis using either the Fusion Tribrid Mass spectrometer and/or one of the 6600 TripleToF mass spectrometers enable the characterisation of the whole protein, including any post-translational modifications.
Protein-protein interactions and protein complex can be analysed using the latest mass spectrometry compatible cross-linking methods and our Tribrid Mass spectrometer platforms.
We have a range of analysis packages for quantitative proteomics analysis, including:
- PeakView/SWATH
- Proteome Discoverer
- Spectronaut
Other types of available proteomics analysis software include:
- Scaffold
- Byonic
- MASCOT
We recommend MaxQuant as an open-source package for quantitative proteomics analysis. The software is available on a mix of high performance workstations (up to 128 core) and virtual computing resources.