Proteomics
Identifying and quantifying complex mixtures of proteins is a huge task. The best platform to use depends on the preferred method of quantitation. For example, Q Exactive HF and Q Exactive HF-X are popular for label free and SILAC analysis. While 6600 TripleTOFs is preferred for SWATH analysis. The Fusion Tribrid mass spectrometer is recommended for optimal TMT labeled based analysis.
Through the use of contemporary enrichment techniques, it is possible to identify and quantify tens of thousands of changes in the phosphoproteome. High throughput methods such as ‘EasyPhos’ are coupled with the latest Q Exactive platforms to create exceptional methods.
Glycomics is the characterisation of polysaccharides, typically derived from proteins. The Orbitrap Velos Pro platform is dedicated for this workflow. In glycopeptidomics, the aim is to identify both the sugar and the peptide while they are still linked together. This can be done on the Velos, or on the Fusion Lumos mass spectrometer which is equipped with Electron Transfer Dissociation and UVPD.
Top-down and intact protein analysis using either the Fusion Tribrid Mass spectrometer and/or one of the 6600 TripleToF mass spectrometers enable the characterisation of the whole protein, including any post-translational modifications.
We have a range of analysis packages for quantitative proteomics analysis, including:
- ProteinPilot
- PeakView/SWATH
- Progenesis QI for Proteomics
- Proteome Discoverer
Other types of available proteomics analysis software include:
- Spectronaut
- Scaffold
- Byonic
- MASCOT
We recommend MaxQuant as an open-source package for quantitative proteomics analysis. The software is available on a mix of high performance workstations, virtual computing resources and network licenses.
A full complement of apparatus for running 2D gels from 7cm to 24cm is available. Progenesis SameSpot software is available for the analysis of 2D gels.
