For enquiries relating to biological specimen preparation, please contact:
There are four Leica Ultracut ultramicrotomes, including the latest model (UC7), available to cut semi-thin (for light microscopy) and ultra-thin (for TEM) sections of resin-embedded biological and materials specimens. High quality glass knives for ultramicrotomy can easily be produced using our Leica KMR3 Knifemaker.
The cryo ultramicrotome enables semi-thin and ultra-thin sectioning of biological specimens and materials samples at a wide at sub-zero temperatures ranging between -15°C to -185°C. Biological specimens can be unfixed, cryoprotected, frozen and sectioned, a method which is particularly useful for immunolabelling of tissue. Often materials samples are too soft section at room temperature, but are able to be cut frozen using the cryo-ultramicrotome.
The Leica EMPACT2 High Pressure Freezer (HPF) is used to rapidly freeze small biological specimens for TEM. The omission of chemicals during fixation greatly improves the preservation of the specimen at the ultrastructural level. Specimens are subjected to very high pressure immediately before being injected with liquid nitrogen, which eliminates the formation of ice crystals, producing vitreous samples. Tissue pieces, liquid suspensions or cell monolayers can be frozen in this way to a depth up to 200 microns.The HPF is also equipped with the following tools:
The Automatic Freeze Substitution unit (AFS) is used in conjunction with the HPF. Frozen specimens are transferred to the pre-cooled AFS unit, where the ice is gradually replaced by solvent or solvent/fixative solutions (eg: acetone or acetone/osmium) at sub-zero temperatures. This process minimises extraction of cell contents during the course of the dehydration and also maintains antigenicity.An average AFS run takes 5 days. There are two Leica AFS units available, including the latest model which is equipped with a robotoic attachment that allows the entire FS process to be carried out fully automatically.
Also known as Vibrating Blade Microtome, this instrument is used to section soft to medium hard specimens without prior embedding in resin or paraffin. Section thickness can be set from 1 to 1000 microns. The vibrating blade reduces compression of the sample during sectioning, allowing samples as soft as brain material to be sectioned. Samples can be fresh or fixed and sections can be collected and stained or immunolabelled for confocal/light microscopy before possible further processing for EM.
The Vitrobot is an automated plunge-freezing instrument used to prepare samples in suspension for cryo-TEM imaging on the JEOL 2100. The sample is placed directly on a TEM grid in a humidity and temperature controlled chamber then rapidly plunge frozen in liquid ethane, preserving ultrastructure as close as possible to the native state. Samples are then viewed under the TEM in cryogenic conditions.
The Critical Point Dryer (CPD) uses liquid carbon dioxide to flush solvent from dehydrated biological specimens to dry them for SEM imaging. This process much gentler than either air-drying or chemical drying with Hexamethyldisilazane (HMDS) and is particularly recommended when processing plant samples for SEM. A typical CPD run takes approximately 2 hours.
This facility is designed to accommodate the culture of mammalian cell lines. We provide the media, components, serum supplements and reagents necessary to sustain cells and tissues in culture systems. There are biological safety cabinets for the sterile handling of cells, as well as several atmosphere-controlled incubators for growing cells. Phase-contrast, fluorescence, standard bright-field microscopes, as well as a cell counter are available for cell analysis.
For enquiries relating to cell culture, please contact:
02 9351 7566
The Class II biological safety cabinet provides protection for personnel, environment and products in work with microorganisms and where the work produces a significant quantity of aerosol. The cabinet provides protection by creating an air barrier at the work opening, recirculating HEPA-filtered laminar airflow downwards through the work zone and by HEPA filtration of exhaust air.
The cytotoxic drug safety cabinet is designed to provide safety protection to the operator, products and the environment against exposure to aerosols and vapours that can be generated in the preparation and manipulation of cytotoxic drugs.
The requirements for protection involve the following:
The ES-315 Autoclave provides convenient processing of your laboratory sterilisation and media preparation needs. The autoclave offers three cycles of operation ranging from basic sterilisation cycles to automatic warming cycles.
The warming cycle may be used directly following a sterilisation cycle or independently to melt and keep sterile media ready for use. The cycles may be programmed up to 99 hours in advance using the Delayed Start Timer. This feature enables you to have sterile media on hand and ready for use when you need it.
To ensure safety and complete sterilisation, the autoclave is equipped with an independent temperature and pressure monitoring system.
This multilabel reader covers all of the main non-radioactive reading technologies. Technologies and reading modes include fluorometry, luminometry, fluorescence polarisation, time-resolved fluorometry (TRF), and photometry (including UV absorbance). The plate reader is also loaded with temperature control and shaking.
HEPA air filtration systems provide continuous protection against unwanted airborne contaminants. The incubators provide precise CO2control with excellent temperature uniformity for researchers seeking the benefits of complete contamination control and dependable long-term performance.
For enquiries relating to materials specimen preparation, please contact:
Materials Specimen Preparation Specialist